Repeated Dose Dermal Toxicity: 21/28-Day Study (OECD TG 410)

TG 410 is an OECD valiated test to identify the health hazard of solid or liquid test substance following dermal application This method is composed of two tests: the main test and the limit test. This Test Guideline is intended for use with the adult rat, rabbit or guinea pig. At least 10 animals (5 female and 5 male) with healthy skin should be used for each of at least three dose levels. The highest dose level should result in toxic effects but not be fatal. The limit test corresponds to one dose level of at least 1000 mg/kg body weight. Test chemical is repeatedly applied for a limited time each day (several hours daily for 21/28 days). The test chemical should be applied over not less than 10 per cent of the body surface area. The results of this study include haematology, clinical biochemistry and urinalysis, gross necropsy and histopathology. A properly conducted 21-day or 28-day study should provide information on the effects of repeated inhalation exposure and can indicate the need for further longer term studies and provide information on the dose levels of the latter.