Repeated Dose 28-Day Oral Toxicity Study in Rodents (OECD TG 407)

TG 407 is an OECD validated method providing information on health hazard likely to arise from exposure to test chemical via oral administration. The method is based on the repeated oral administration of the chemical of interest during one limited period (one dose level daily during 28 days). This Guideline is intended primarily for use with rodents (rat preferably). At least 10 animals (5 female and 5 male) should be used for each dose level. Three tests groups, at least, should be used. The test chemical is administered by gavage or via the diet or drinking water. A limit test may be performed if no effects would be expected at a dose of 1000 mg/kg bw/d. The report of this study will include results from clinical and functional observations, body weight and food/water consumption measurements, hematology and clinical biochemistry as well as gross necropsy and histopathology.