Non-OECD non-mammalian screens and tests (Conceptual Framework Levels 3-5)

Specific guidance is provided for in vivo screens and tests using non-mammalian vertebrates and invertebrates for evaluating endocrine disrupting chemicals. The assays in this section have not been fully validated as OECD test guidelines, but have undergone partial validation (Androgenised Female Stickleback Screen (GD 148), Harpacticoid Copepod Development and Reproduction Test with Amphiascus (GD 201)) have been validated at the national level ( Fish Life Cycle Toxicity Test (FLCTT), Avian Two-Generation Toxicity Test in the Japanese Quail (ATGT)) are draft test guidelines in the OECD validation process (Short-Term Juvenile Hormone Activity Screening Assay using Daphnia magna, EASZY assay for detection of substances acting through estrogen receptors using zebrafish embryos, Juvenile Medaka Anti-Androgen Screening Assay, Xenopus Embryonic Thyroid Signalling Assay (XETA), Zebrafish Extended One-Generation Reproduction Test (ZEOGRT), RADAR: Rapid Androgen Disruption Adverse Outcome Reporter Assay) or are assays proposed as OECD test guidelines (Daphnia Multigeneration Test for Assessment of Endocrine-Active Chemicals (DMGT)) at the time of publication of this document.