Daphnia magna Reproduction Test (OECD TG 211)

TG 211 in an OECD validated test that assesses the effect of chemicals on the reproductive output of Daphnia magna Straus. To this end, young female Daphnia are exposed to the test chemical added to water at a range of concentrations (at least five). For semi-static tests, at least 10 animals at each test concentration and for flow-through tests, 40 animals divided into four groups of 10 animals at each test concentration are used. The test duration is 21 days. The total number of living offspring produced per parent animal and the number of living offspring produced per surviving parent animal at the end of the test are reported. The study report also includes: the daily counting of the offspring, the daily recording of the parent mortality, the weekly measurement of oxygen concentration, temperature, hardness and pH values and the determination of the concentrations of test substance. Optionally other effects can be reported, including the sex ratio of the offspring.